pub110 plasmid Molecular

Bacillus subtilis DB428,DB430 醱酵系統 …

The plasmid stability studies demonstrated that pUB110 was the most stable plasmid whereas pE194 was the lowest stable plasmid. However, medium with different nitrogen and carbon sources did not affect sporulation of B. subtilis DB428 and DB430.

Inactivation of aprE Gene in Bacillus subtilis 168 by Homologus …

 · PDF 檔案plasmid (pUB110) and insert were mixed for liga-tion reaction with T4 DNA ligase. Then recombi-nant plasmid was transformed into B. subtilis 168. Figure 1 shows the pUB110plasmid digested with EcoRI and XbaI and also the 80 bp insert. The integrity of thekb

Strategy To Approach Stable Production of …

The pUB110/pUC‐based plasmid was found to exhibit high structural instability with the identification of a DNA deletion between two repeated regions. An initial attempt was made to eliminate the homologous site in the plasmid, whereas the stability of the resulting plasmid was not improved.
Frontiers
P, purified plasmid DNA 15 ng (pBG55 in A, and pUB110 in B); C, control, a SPP1 infection of BG214 cells without plasmid. We also observed the appearance of a similar 44-kb plasmid band after infection with the wt SPP1 phage of cells bearing the natural pUB110 plasmid (Figure 3B ).

XYLANASES FROM BACILLUS SUBTILIS EXPRESSED IN B. SUBTILIS

 · PDF 檔案was inserted into the pUB110 plasmid by replacing a large portion of the mobilization gene. Therefore, the mobilization gene could not be expressed and the plasmid could not be tramsferred to other B. subtilis strains. Such modified vector was then introduced

US Patent for Mutant microorganisms containing …

Recombinant DNA containing amylase-coding genes is prepared by cleaving DNA from various donor microorganisms and combining portions of the DNA with the plasmid pUB110. Strains of E. coli or B. subtilis containing the recombinant DNA are grown in

Transformation of heat-treated Clostridium …

The U.S. Department of Energy’s Office of Scientific and Technical Information OSTI.GOV Journal Article: Transformation of heat-treated Clostridium acetobutylicum protoplasts with pUB110 plasmid DNA

Enrichment of plasmid DNA in cell membranes of Bacillus …

Abstract The discrepancy between previously reported copy numbers for the plasmid pUB110 in Bacillus subtilis and the copy number determined by nucleic acid sandwich hybridization of a pUB110‐d

Inter- and Intraspecies Conjugal Transfer of Different …

Conjugal transfer of plasmid pUB110 between different strains of bacilli was studied. The plasmid transfer was possible not only between various strains of B. subtilis, but also when many other species of bacilli served as recipients. Conjugation of a donor strain B. subtilis 19 (p19 pUB110) was accompanied by a transfer of plasmid p19 along with plasmid pUB110 to the B. subtilis recipient

[Hybrid vectors for the cyanobacterium Anacystis …

The plasmid pBS20, containing the complete sequence of pANS and pUB110 DNA, transforms Bacillus subtilis rec E4 protoplasts being, however, unstable in bacilli cells and disintegrates deriving a parent pUB110 plasmid.

Vaccination against Anthrax with Attenuated …

While plasmid copy number was not measured, pUB110 is known to be a multicopy plasmid in B. subtilis (), whereas pX01 is present in only one or two copies per cell of B. anthracis (). In addition, pPA102 has undergone a spontaneous deletion which may have removed a negative regulatory region upstream of the PA gene ( 20 ).

Purification and characterization of a kanamycin …

Sadaie, Y, Burtis, KC & Doi, RH 1980, ‘ Purification and characterization of a kanamycin nucleotidyltransferase from plasmid pUB110-carrying cells of Bacillus subtilis ‘, Journal of Bacteriology, vol. 141, no. 3, pp. 1178-1182.
PRIME PubMed
The Bacillus subtilis plasmid pKBT1, the product of in vivo recE4-independent recombinal events, contains segments derived from pUB110 and the B. subtilis chromosome. To determine whether the pUB110 sequence is intact in PKBT1, two 1 kb fragments, each containing a site at which chromosomal and pUB110 sequences are joined, were cloned and sequenced.
(PDF) Chromosomal aadD2 Encodes an Aminoglycoside Nucleotidyltransferase in Bacillus clausii

Molecular cloning of genetically active fragments of …

Plasmid pUB110 (approximately 2.8 x 10(6) daltons), originally detected in Staphylococcus aureus, specifies resistance to neomycin and has been transformed into Bacillus subtilis 168, In B. subtilis, pUB110 is stably maintained at about 50 copies per chromosome and renders the host resistant to neomycin sulfate at 5 microgram/ml. pUB110 isolated from B. subtilis transforms Rec+ and recE4

New Shuttle Vector-Based Expression System To Generate Polyhistidine-Tagged Fusion Proteins …

 · PDF 檔案copy numbers, and plasmid instability (9–11). While some sso sequences support replication only in the native host, other se-quences are functional in a number of different Gram-positive bacteria (8). For example, pUB110 is a broad-host-range plasmid whose

Rolling-Circle Replication of Bacterial Plasmids

 · PDF 檔案pT181 family (and possibly the pUB110 plasmid), is that the replication fork proceeds approximately 10 nucleotides (nt) beyond the Rep nick site and the hairpin structure surrounding the nick site is regenerated. At this stage, interaction of the Rep protein with

Differential transfer dynamics of pAW63 plasmid …

Aim: To assess the dynamics of plasmid transfer between Bacillus thuringiensis and B. cereus in various food microcosms using the B. thuringiensis pAW63 and Staphylococcus aureus pUB110 plasmids as models. Methods and Results: The conjugative behaviour of pAW63, which resembles the B. anthracis virulence plasmid pXO2, and the mobilization of pUB110 were investigated using kinetics studies

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